Journal
LABORATORY ANIMALS
Volume 40, Issue 3, Pages 275-281Publisher
ROYAL SOC MEDICINE PRESS LTD
DOI: 10.1258/002367706777611479
Keywords
catheterization; infusion; metabolic phenotyping; mouse models; stable isotope
Categories
Funding
- NIDDK NIH HHS [DK54450] Funding Source: Medline
Ask authors/readers for more resources
We have developed and validated catheterization protocols in mice that allow for simultaneous infusion and sampling. A sampling catheter was inserted in the lateral vein of the tail, while the animals were infused either intravenously or intragastrically through a second catheter placed in the contralateral lateral vein or via an intragastric catheter, respectively. The applicability of these methods of infusion and blood sampling were validated by conducting urea kinetics utilizing stable isotopes. These non-surgical procedures are non-invasive, inexpensive, fast to perform and animals do not require a recovery period before their use.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available