Journal
MICROBIAL PATHOGENESIS
Volume 41, Issue 1, Pages 43-47Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micpath.2006.04.004
Keywords
Borrelia burgdorferi; Spirochete; Gene fusion; Lyme disease
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Funding
- NIAID NIH HHS [T32 AI49795, R01 AI44252] Funding Source: Medline
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A novel, infectious Borrelia burgdorferi that expresses green fluorescent protein (GFP) was developed to examine the utility of this marker protein to label live bacteria during infection processes. Use of a borrelial erpAB promoter to direct gfp transcription supported previous indications that B. burgdorferi expresses erp genes during chronic mammalian infection and during acquisition by feeding ticks. Live bacteria fluoresced and were seen to be located extracellularly in infected mice and within midguts of infected ticks. These results indicate that transcriptional fusions between B. burgdorferi promoters and gfp can be useful tools to examine spirochete gene expression in vivo. (c) 2006 Elsevier Ltd. All rights reserved.
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