Journal
ELECTROPHORESIS
Volume 27, Issue 13, Pages 2590-2608Publisher
WILEY
DOI: 10.1002/elps.200600047
Keywords
affinity capillary electrophoresis; binding studies; capillary electrophoretic mobility shift analysis; DNA; frontal analysis
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The versatility of CE is beneficial for the study of many types of molecular interactions, because different experimental designs can be made to suit the characteristics of a particular interaction. A very versatile starting point is the preequilibration type of affinity CE that has been used extensively for characterizing biomolecular interactions in the last 15 years. We review this field here and include a comprehensive overview of the existing preincubation ACE modes including their advantages and limitations as well as the methodological developments and applications within the bioanalytical field.
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