Journal
ARTHRITIS AND RHEUMATISM
Volume 54, Issue 7, Pages 2164-2174Publisher
WILEY
DOI: 10.1002/art.21941
Keywords
-
Categories
Funding
- NIAMS NIH HHS [AR-48182, AR-15768, AR-50245] Funding Source: Medline
Ask authors/readers for more resources
Objective. To determine whether interleukin-1 (IL-1) initiates transient changes in the intracellular concentration of [Ca2+](i) and the organization of filamentous actin (F-actin) in articular chondrocytes. Methods. Articular chondrocytes within cartilage explants and enzymatically isolated chondrocytes were loaded with Ca2+-sensitive fluorescence indicators, and [Ca2+](i) was measured using confocal fluorescence ratio imaging during exposure to 10 ng/ml IL-1 alpha. Inhibitors of Ca2+ mobilization (Ca2+-free medium, thapsigargin [inhibitor of Ca-ATPases], U73122 [inhibitor of phospholipase C], and pertussis toxin [inhibitor of G proteins]) were used to determine the mechanisms of increased [Ca2+](i). Cellular F-actin was quantified using fluorescently labeled phalloidin. Toxin B was used to determine the role of the Rho family of small GTPases in F-actin reorganization. Results. In isolated cells on glass and in in situ chondrocytes within explants, exposure to IL-1 induced a transient peak in [Ca2+](i) that was generally followed by a series of decaying oscillations. Thapsigargin, U73122, and pertussis toxin inhibited the percentage of cells responding to IL-I. IL-1 increased F-actin content in chondrocytes in a manner that was inhibited by toxin B. Conclusion. Both isolated and in situ chondrocytes respond to IL-1 with transient increases in [Ca2+](i) via intracellular Ca2+ release mediated by the phospholipase C and inositol trisphosphate pathways. The influx of Ca2+ from the extracellular space and the activation of G protein-coupled receptors also appear to contribute to these mechanisms. These findings suggest that Ca2+ mobilization may be one of the first signaling events in the. response of chondrocytes to IL-1.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available