Journal
CELL MOTILITY AND THE CYTOSKELETON
Volume 63, Issue 7, Pages 415-422Publisher
WILEY-LISS
DOI: 10.1002/cm.20133
Keywords
inflammation; leukocyte; chemotaxis; microtubutes; Rho kinase; cell motility
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Funding
- Medical Research Council [G0401026] Funding Source: researchfish
- MRC [G0401026] Funding Source: UKRI
- Medical Research Council [G0401026] Funding Source: Medline
- Wellcome Trust Funding Source: Medline
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The inflammatory response is one of the most dramatic examples of directed cell movement in nature. Inflammation is triggered at the site of injury and results in the migration of immune cells to the site to protect the host from infection. We have devised an in vivo inflammation assay using translucent zebrafish embryos, which allow live imaging and pharmacological manipulation of macrophage chemotaxis to wounds inflicted with a laser. Using this assay, we test the role of the microtubule cytoskeleton in macrophage chemotaxis in vivo using nocodazole to disrupt microtubule polymerization. We find that de-stabilisation of microtubules with nocodazole impairs macrophage recruitment to wounds, but that addition of the Rho kinase inhibitor Y-27632 suppresses these effects and restores the recruitment of macrophages to wounds. Taken together, these results suggest that destabilizing microtubules activates Rho kinase and that this increase in Rho kinase activity interferes with leukocyte recruitment in vivo.
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