Journal
INFECTION AND IMMUNITY
Volume 74, Issue 7, Pages 4142-4148Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/IAI.00299-06
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Funding
- NIAID NIH HHS [R01 AI047499, R01 AI47499] Funding Source: Medline
- NIDDK NIH HHS [R01 DK52081, R01 DK052081] Funding Source: Medline
- NIGMS NIH HHS [T32 GM007270, GM07270] Funding Source: Medline
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Escherichia coli O157:H7 is a commensal organism in cattle, but it is a pathogen in humans. This differential expression of virulence suggests that specific virulence factors are regulated differently in human and bovine hosts. To test this hypothesis, relative real-time reverse transcription-PCR was used to relate the expression of several putative virulence genes (eae espA stx(2) rfbE, ehx4, and iha) to that of the housekeeping gene gnd during natural human and experimental bovine infection with E. coli O157:H7. We examined these genes in fecal samples from eight humans and four calves. iha and espA were significantly more expressed in bovine infections. rfbE and ehxA appeared to be more highly expressed in human infections, though these differences did not achieve statistical significance. Our results support the hypothesis that some virulence-associated genes of O157:H7 are differentially expressed in a host-specific manner.
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