Journal
PLANT CELL REPORTS
Volume 25, Issue 7, Pages 636-642Publisher
SPRINGER
DOI: 10.1007/s00299-006-0129-2
Keywords
Cedrus; DNA methylation; in vitro storage; minimal growth; RAPD
Categories
Ask authors/readers for more resources
We developed procedures for slow-growth storage of Cedrus atlantica and Cedrus libani microcuttings of juvenile and adult origin, noting factors favouring the extension of subculture intervals. Microcuttings could be stored effectively up to 6 months at 4 degrees C and reduced light intensity, provided that they were grown on a diluted modified MS medium. The addition of 6% mannitol to the storage media affected negatively survival and multiplication capacity of the cultures. The slow-growth storage conditions used in our experiments did not induce remarkable effects on both RAPD variability and average DNA methylation in the species.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available