Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 11, Issue 4, Pages -Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.2343208
Keywords
dehydration; optical clearing; density; scattering coefficient; refractive index; cell organelle; collagen fibril; optical coherence tomography; transmission electron microscopy
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Previous studies identified various mechanisms of light scattering reduction in tissue induced by chemical agents. Our results suggest that dehydration is an important mechanism of optical clearing in collagenous and cellular tissue. Photographic and optical coherence tomography images indicate that air-immersed skin and tendon specimens become similarly transparent to glycerol-immersed specimens. Transmission electron microscopy images reveal that dehydration causes individual scattering particles such as collagen fibrils and organelles to become more densely packed, but does not significantly alter size. A heuristic particle-interaction model predicts that the scattering particle volume fraction increase can contribute substantially to optical clearing in collagenous and cellular tissue. (c) 2006 Society of Photo-Optical Instrumentation Engineers.
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