Journal
ANNALS OF MICROBIOLOGY
Volume 63, Issue 4, Pages 1571-1577Publisher
BMC
DOI: 10.1007/s13213-013-0620-2
Keywords
Anaerobic rumen fungi; Fungal diversity; ITS1; Cattle; Orpinomyces
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Funding
- National Agricultural Innovation Project, Indian Council of Agricultural Research, New Delhi, India
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Fiber degradation in the ruminant digestive process is a major activity accomplished by rumen microbes, a process in which the role of fungi is important. Therefore, the present study was conducted to establish the community structure of anaerobic rumen fungi in cattle fed on a high fiber diet using molecular approaches. Total community DNA was extracted, and the ribosomal internal transcribed spacer (ITS) 1 region was amplified, cloned, and sequenced. The resulting nucleotide sequences were used to construct a phylogenetic tree. A total of 52 clones were analyzed, revealing 31 different ITS1 gene phylotypes. Of these, 12 belonged to the genus Orpinomyces (48 % of clones), followed by uncultured Neocallimastigale clones (29 %), Cyllamyces spp. (9 %) and Anaeromyces spp. (8 %). Our results indicate that genus Orpinomyces dominates the rumen fungal community in Indian crossbred Karan Fries cattle.
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