Journal
MATRIX BIOLOGY
Volume 25, Issue 5, Pages 317-320Publisher
ELSEVIER
DOI: 10.1016/j.matbio.2006.03.006
Keywords
ADAMTS4; synovium; alternative splice; osteoarthritis
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The major proteoglycan of articular cartilage aggrecan is a substrate for ADAMTS4. RT-PCR analysis of human osteoarthritic (OA) synovial co-cultures using oligonucleotide primers designed to amplify across the exon 8/9 junction of human ADAMTS4 resulted in the amplification of two products, the expected product and a smaller product missing 161 bp from the 5' end of exon 9, the result of alternative splicing in which exon 8 joins to a cryptic 3' splice site within exon 9. The protein produced would be identical to human ADAMTS4 up to Arg(696), and would have a new C-terminal domain with no commonality with the ADAMTS4 spacer domain. Changes in the C-terminal domain of ADAMTS4 may alter its substrate specificity. (c) 2006 Elsevier B.V./Intemational Society of Matrix Biology. All rights reserved.
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