Chryseobacterium wanjuense sp nov., isolated from greenhouse soil in Korea

A taxonomic study was performed on strain R2A10-2(T) isolated from greenhouse soil cultivated with lettuce (Lacturca sativa L.) collected in Wanju Province, Korea. The bacterial cells were Gram-negative, aerobic, short rods. The growth temperature and pH were 5-35 degrees C and 5(.)0-9(.)0, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that this isolate had 93-3-97-7% similarity to Chryseobacterium species: the highest sequence similarities were to the type strains of Chryseobacterium daecheongense (97(.)7%), Chryseobacterium formosense (97(.)1%) and Chryseobacterium defluvii (96(.)9%). Low levels of DNA-DNA relatedness were found between strain R2A10-2(T) and the type strains of these three species (< 28%). Differences in phenotypic properties were found with respect to Chryseobacterium species with validly published names. The predominant cellular fatty acids were iso-15:0 (40(.)0%), iso-17:0 3-OH (21(.)9%), iso-17 : 1 omega 9c (11(.)7%) and summed feature 4 (iso-15:0 2-OH and/or 16 : 1 omega 7c/t, 11(.)0%). Menaquinone IMK-6 was detected as the sole respiratory quinone. The G + C content of the genomic DNA was 37(.)8 mol%. On the basis of the genomic and phenotypic evidence, this isolate represents a novel species of the genus Chryseobacterium, for which the name Chryseobacterium wanjuense sp. nov. is proposed. The type strain is R2A10-2(T) (= KACC 11468(T) = DSM 17724(T)).