Journal
NATURE CELL BIOLOGY
Volume 8, Issue 7, Pages 668-U54Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/ncb1424
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Funding
- NIGMS NIH HHS [GM71747, GM58065, GM21841] Funding Source: Medline
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The DExD/H-box ATPase Dbp5 is essential for nuclear mRNA export, although its precise role in this process remains poorly understood. Here, we identify the nuclear pore protein Gle1 as a cellular activator of Dbp5. Dbp5 alone is unable to stably bind RNA or effectively hydrolyse ATP under physiological conditions, but addition of Gle1 dramatically stimulates these activities. A gle1 point mutant deficient for Dbp5 stimulation in vitro displays an mRNA export defect in vivo, indicating that activation of Dbp5 is an essential function of Gle1. Interestingly, Gle1 binds directly to inositol hexakisphosphate ( InsP(6)) and InsP(6) potentiates the Gle1-mediated stimulation of Dbp5. Dominant mutations in DBP5 and GLE1 that rescue mRNA export phenotypes associated with the lack of InsP(6) mimic the InsP(6) effects in vitro. Our results define specific functions for Gle1 and InsP(6) in mRNA export and suggest that local activation of Dbp5 at the nuclear pore is critical for mRNA export.
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