Journal
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
Volume 48, Issue 7, Pages 823-831Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.1744-7909.2006.00281.x
Keywords
non-invasive microsensing system (NMS); oxygen fluxes; pollen tube; proton fluxes; scanning ion-selective electrode technique (SIET); scanning polarographic electrode technique (SPET); two-probe simultaneous test system (2STS)
Categories
Ask authors/readers for more resources
Various ionic and molecular activities in the extracellular environment are vital to plant cell physiological processes. A noninvasive microsensing system (NMS) based on either the scanning ion-selective electrode technique (SIET) or the scanning polarographic electrode technique (SPET) is able to obtain information regarding the transportation of various Ions/molecules in intact samples under normal physiological conditions. The two-probe simultaneous test system (2STS) Is an integrated system composed of SIET, SPET, and a Xu-Kunkel sampling protocol. In the present study, 2STS was able to simultaneously measure fluxes of H(+) and O(2) of the lily (Lillum longiflorum Thunb. cv. Ace) pollen tube while avoiding interference between the two probes. The results Indicate that the proton fluxes were effluxes, whereas the oxygen fluxes were influxes, and they were closely correlated to each other surrounding the constitutive alkaline band region. Specifically, when the proton effluxes increased, the oxygen influxes also increased. Therefore, the hypothesis of condensed active mitochondria existing in the alkalized area of the pollen tube proposed by Hepler's group is supported.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available