Journal
BIOCHEMISTRY-MOSCOW
Volume 71, Issue 7, Pages 815-820Publisher
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S0006297906070157
Keywords
restriction-modification system; restriction endonuclease; site-specific DNA nickase; heterodimeric restriction endonuclease
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We are the first to have isolated a protein (186 amino acid residues) encoded by the open reading frame adjacent to the end of the BspD6I nickase (N.BspD6I) gene. Cleavage of both DNA strands near the sequence recognized by nickase (5'-GAGTC/5'-GACTC) occurs when this protein is added to the reaction mixture containing N.BspD6I. The protein encoded by the open reading frame and the nickase are suggested to be subunits of heterodimeric restriction endonuclease R.BspD6I.
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