Journal
PLANT SCIENCE
Volume 171, Issue 1, Pages 99-105Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2006.02.017
Keywords
Zea mays L.; kinase activity; abiotic stress; salicylic acid; phosphorylation
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Pti1 plays an important role in plant disease responses. One full-length cDNA encoding a Pti I homologue was isolated from maize by RT-PCR and named as ZmPti1. The ZmPti1 protein was expressed in Saccharomyces cereviciae, purified by ProBond Resin and examined for its autophosphorylation ability. The phosphorylation assay in vitro showed that ZmPti1 has a kinase activity. RT-PCR analysis indicated that the ZmPti1 expression is induced by salicylic acid (SA), low-temperature, mannitol and salt, but not by abscisic acid (ABA). Furthermore, in different tissues the ZmPti1 displays different expression patterns. These results demonstrate that ZmPti1 is a novel Pti1-like gene and may play multiple roles in both plant biotic and abiotic resistance pathways. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
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