4.4 Article

Mapping novel traits by array-assisted bulk segregant analysis in Saccharomyces cerevisiae

Journal

GENETICS
Volume 173, Issue 3, Pages 1813-1816

Publisher

GENETICS SOCIETY AMERICA
DOI: 10.1534/genetics.106.057927

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Funding

  1. NHGRI NIH HHS [F32 HG002649, F32 HG02649-01] Funding Source: Medline
  2. NIGMS NIH HHS [P50 GM071508, R01 GM046406] Funding Source: Medline

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We demonstrate a new method, microarray-assisted bulk segregant analysis, for mapping traits in yeast by genotyping pooled segregants. We apply a probabilistic model to the progeny of a single cross and as little as two microarray hybridizations to reliably map an auxotrophic marker, a Mendelian trait, and a major-effect quantitative trait locus.

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