Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1120, Issue 1-2, Pages 230-236Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2005.11.109
Keywords
Scutellaria L. genus; solid-phase extraction; flavones; chemotaxonomic markers
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In this study, the effectiveness of both classical and novel polymeric sorbents used in solid-phase extraction (SPE) for isolation of pharmacologically active flavones (baicalin, luteolin, apigenin, wogonin and chrysin) from aerial parts of 13 species of Scutellaria L. (Skullcap) genus was assessed. The highest recoveries of hydrophilic (baicalin, luteolin) flavones for Oasis (R) HLB were obtained, whereas for medium hydrophobic (apigenin) and hydrophobic (wogonin, chrysin) flavones better quantitative results for BakerBond phenyl cartridges were stated. Eluates were analysed using reversed-phase high-performance liquid chromatography with diode array detection (RP-HPLC-DAD). For the five target compounds the determined concentrations ranged from similar to 4 to similar to 15,500 mu g/g dry wt. Very good linearities (r(2) > 0.9995) of calibration curves were achieved for each flavone. The accuracy was below 5% for most compounds examined. This is the first method reported that enabled simultaneous qualification and quantitation of five flavones (being chemotaxonomic markers) in 13 species of Scutellaria L. genus. (c) 2005 Elsevier B.V. All rights reserved.
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