Journal
ANALYTICA CHIMICA ACTA
Volume 571, Issue 2, Pages 200-205Publisher
ELSEVIER
DOI: 10.1016/j.aca.2006.04.071
Keywords
fibrinogen; immune resonance scattering spectral assay; gold nanoparticle label
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Gold nanoparticles in size of 9.0 nm was prepared by the trisodium citrate and used to label goat anti-human fibrinogen. In the pH 6.2 buffer solution and in the presence of polyethylene glycol (PEG), the immune reaction between gold-labeled goat anti-human fibrinogen and fibrinogen took place and the labeled gold nanoparticles were released from the goat anti-human fibrinogen, and the released gold particles aggregated which leaded the resonance scattering intensity at 560 nm (I-560mm) to enhance greatly. The I-560mm is proportional to the fibrinogen concentration in the range from 0.027 to 1.07 mu g mL(-1). The detection limit is 1.14 ng mL(-1). This simple assay was applied to determination of fibrinogen in human plasma, with satisfactory results. (c) 2006 Elsevier B.V. All rights reserved.
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