Journal
CANCER LETTERS
Volume 238, Issue 1, Pages 53-60Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2005.06.022
Keywords
puerarin; apoptosis; human colon cancer HT-29 cell line; Bcl-2; c-myc; caspase-3
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Puerarin was isolated from Pueraria radix and has beneficial effects on cardiovascular, neurological, and hyperglycemic disorders. The current study showed that puerarin also possessed anti-cancer properties. Methyl thiazolyl tetrazolium assay (MTT) assay revealed a dose-dependent reduction of HT-29 cellular growth in response to puerarin treatment. Apoptosis was observed following treatments 'with >= 25 mu M puerarin, as reflected by the appearance of the subdiploid fraction and NDA fragmentations. We then investigated effects of puerarin on expression of apoptosis-associated genes and the results revealed an increase of bax and decreases of c-myc and bcl-2. Finally, puerarin treatment significantly increased the activation of caspase-3, a key executioner of apoptosis. These findings indicate that puerarin may act as a chemopreventive and/or chemotherapeutic agent in colon cancer cells by reducing cell viability and inducing apoptosis. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
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