Journal
FEBS LETTERS
Volume 580, Issue 16, Pages 3900-3904Publisher
WILEY
DOI: 10.1016/j.febslet.2006.06.019
Keywords
bisphenol; intracellular calcium; invertebrate fertilization; PMCA; SPCA
Funding
- NICHD NIH HHS [HD12986] Funding Source: Medline
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Plasma membrane, sarco-endoplasmic reticulum and secretory pathway Ca2+-ATPases (designated PMCA, SERCA and SPCA) regulate intracellular Ca2+ in animal cells. The presence of PMCA, and the absence of SERCA, in sea urchin sperm is known. By using inhibitors of Ca2+-ATPases, we now show the presence of SPCA and Ca2+ store in sea urchin sperm, which refills by SPCA-type pumps. Immunofluorescence shows SPCA localizes to the mitochondrion. Ca2+ measurements reveal that similar to 75% of Ca2+ extrusion is by Ca2+ ATPases and 25% by Na+ dependent Ca2+ exchanger/s. Bisphenol, a Ca2+ ATPase inhibitor, completely blocks the acrosome reaction, indicating the importance of Ca+-ATPases in fertilization. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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