Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 838, Issue 2, Pages 107-112Publisher
ELSEVIER
DOI: 10.1016/j.jchromb.2006.04.020
Keywords
pyridoxal 2-chlorobenzoyl hydrazone; o-108; iron chelator; HPLC; pharmacokinetics
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A high performance liquid chromatographic method for the determination of a biocompatible iron chelator, pyridoxal 2-chlorobenzoyl hydrazone (o-108), in rabbit plasma was developed and validated. The separation was achieved on a C 18 column with the mobile phase composed of a mixture of 0.01 M phosphate buffer (pH 6) with the addition of EDTA (2 mM), methanol and acetonitrile (42:24:14;v/v/v). The method was validated with respect to selectivity, linearity (0.8-150 mu g/mL), intra- and inter-day variability and stability. This method was successfully applied to the analysis of the samples obtained from a pilot pharmacokinetic experiment, in which the chelator was administered intravenously to rabbits. (c) 2006 Elsevier B.V. All rights reserved.
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