Journal
NANO LETTERS
Volume 6, Issue 7, Pages 1318-1324Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nl052458a
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Funding
- NIBIB NIH HHS [7 R01 EB000832] Funding Source: Medline
- NIGMS NIH HHS [GM R01 54008] Funding Source: Medline
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Nanometal surface energy transfer (NSET), which describes an energy transfer event from optically excited organic fluorophores to small metal nanoparticles, may be used as a molecular beacon/ruler similar to FRET, but with advantages over this classical technique. Here we use NSET to measure Mg2+-induced conformational changes for a hammerhead ribozyme and confirm these measurements using FRET. These optical experiments enhance our understanding of the different kinetic pathways for this ribozyme.
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