Journal
JOURNAL OF MOLECULAR BIOLOGY
Volume 360, Issue 3, Pages 523-536Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2006.05.032
Keywords
MSH2-MSH3; mismatch repair; recombination; double-strand break repair
Categories
Funding
- NIGMS NIH HHS [GM-53085, R01 GM053085] Funding Source: Medline
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Genetic studies in Saccharomyces cerevisiae predict that the mismatch repair (MMR) factor MSH2-MSH3 binds and stabilizes branched recombination intermediates that form during single strand annealing and gene conversion. To test this model, we constructed a series of DNA substrates that are predicted to form during these recombination events. We show in an electrophoretic mobility shift assay that S. cerevisiae MSH2-MSH3 specifically binds branched DNA substrates containing 3' single-stranded DNA and that ATP stimulates its release from these substrates. Chemical footprinting analyses indicate that MSH2-MSH3 specifically binds at the double-strand/single-strand junction of branched substrates, alters its conformation and opens up the junction. Therefore, MSH2-MSH3 binding to its substrates creates a unique nucleoprotein structure that may signal downstream steps in repair that include interactions with MMR and nucleoticle excision repair factors. (c) 2006 Elsevier Ltd. All rights reserved.
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