Journal
CANCER RESEARCH
Volume 66, Issue 14, Pages 7095-7102Publisher
AMER ASSOC CANCER RESEARCH
DOI: 10.1158/0008-5472.CAN-06-0515
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Funding
- NCI NIH HHS [R01 CA064786-09, 2 R01 CA064786-09, R01 CA064786] Funding Source: Medline
- NIAID NIH HHS [U19 AI057319, U19-AI057319] Funding Source: Medline
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Nonmalignant human mammary epithelial cells (HMEC) seeded in laminin-rich extracellular matrix (IrECM) form polarized acini and, in doing so, transit from a disorganized proliferating state to an organized growth-arrested state. We hypothesized that the gene expression pattern of organized and growth-arrested HMECs would share similarities with breast tumors with good prognoses. Using Affymetrix HGU133A microarrays, we analyzed the expression of 22,283 gene transcripts in 184 (finite life span) and HMT3522 S1 (immortal nonmalignant) HMECs on successive days after seeding in a IrECM assay. Both HMECs underwent growth arrest in G(0)-G(1) and differentiated into polarized acini between days 5 and 7. We identified gene expression changes with the same temporal pattern in both lines and examined the expression of these genes in a previously published panel of microarray data for 295 breast cancer samples. We show that genes that are significantly lower in the organized, growth-arrested HMEC than in their proliferating counterparts can be used to classify breast cancer patients into poor and good prognosis groups with high accuracy. This study represents a novel unsupervised approach to identifying breast cancer markers that may be of use clinically.
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