Journal
ANALYTICAL BIOCHEMISTRY
Volume 354, Issue 2, Pages 169-174Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2006.04.029
Keywords
quantum dot; water-soluble; conjugate; immunofluorescent labeling; cellular imaging
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Thioglycolic-acid-stabilized CdTe quantum dots. synthesized directly in aqueous solution. are successfully conjugated with biotin and polyethylene glycol. Using these conjugates, we report the development of this kind of water-soluble quantum dot for immunofluorescent labeling of cancer cells. The results show that these conjugates have very low nonspecific binding and good stability against photobleaching, enabling them to be applied in many biological fields, such as cellular labeling, intracellular tracking. and other imaging applications. (c) 2006 Elsevier Inc. All rights reserved.
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