Journal
IMMUNOLOGY LETTERS
Volume 106, Issue 1, Pages 48-56Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.imlet.2006.04.006
Keywords
CD160; CD8(+) memory T cells; intestinal intraepithelial lymphocytes
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CD160 is an Ig-like glycoprotein expressed on NK, NKT and TCRy delta T cells, as well as intestinal intraepithelial T lymphocytes. In addition, a minor subset of CD8(+) but not CD4(+) T cells in the periphery is also known to express CD160, but the subset has not been fully characterized. In this study, we prepared anti-murine CD160 mAbs and investigated the expression profile of CD160 on various subsets of CD8(+) T cells. The amount of CD160 on almost all CD8(+) T cells was increased upon CD3-mediated stimulation in vitro, and soluble CD160 was found to be released. Flow cytometrie analysis revealed most CD8(+) T cells expressing CD160 to show a CD44(high) phenotype in vivo. On further analysis, both CD44(high)CD62L(low) effector memory T cells (T-EM) and CD44(high) CD62(high) central memory T cells (T-CM) expressed CD160 at an intermediate level. High levels were evident with recently activated CD8+ T-EM. Naive CD8(+) T cells presumably immediately after stimulation (CD44(low)CD62(low)CD69(+)) also expressed CD160, but only at a low level. Purified CD160(+) CD8(+) T cells from OT-1 transgenic mice expressing TCR against OVA residues 257-264 presented by H-2K(b) produced IFN-gamma more rapidly than CD160- CD8(+) T cells upon antigen stimulation. These results together show that CD160 is expressed on the majority of CD8(+) memory T cells as well as recently activated CD8(+) T cells. (c) 2006 Elsevier B.V. All rights reserved.
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