Journal
ANALYTICAL BIOCHEMISTRY
Volume 354, Issue 2, Pages 255-265Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2006.04.018
Keywords
miniaturized asymmetrical flow-field flow fractionation; particle size; lipoproteins; aggregation and fusion
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Asymmetrical flow field-flow fractionation (AsFIFFF), a technique that provides direct measurement of particle size and diffusion coefficient, is converted into miniaturized scale. In comparison with conventional AsFIFFF, the separation of proteins in miniaturized AsFIFFF is achieved within shorter time periods, with smaller sample amounts, and with lower mobile phase consumption. Minimization of the overloading and optimization of the separation efficiency are prerequisites to good results. Miniaturized AsFIFFF is applied to the measurement of particle sizes of high-density lipoprotein (HDL). low-density lipoprotein (LDL), and very low-density lipoprotein (VLDL). The average hydrodynamic diameters at pH 7.4 in 8.5 mM phosphate buffer containing I mM EDTA and 150 mM NaCl are 8.6 +/- 0.5, 11.2 +/- 10.2, 22.1 +/- 0.7. and 48.9 +/- 7.5 nm for subgroups HDL3. HDL2. LDL, and VLDL, respectively. In addition. the effect of different factors on the aggregation and fusion of LDL particles is studied. LDL particle sizes are unaffected by the addition of up to 300 mM NaCl and by an increase of the carrier solution pH from 3.2 to 7.4. but treatment of LDL with alpha-chymotrypsin, sphingomyelinase, or copper sulfate leads to the formation of aggregated and fused LDL particles. (c) 2006 Elsevier Inc. All rights reserved.
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