Journal
CANCER LETTERS
Volume 238, Issue 2, Pages 260-270Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2005.07.018
Keywords
colon cancer; EGCG; Ras; transformation; cell proliferation
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Funding
- NCI NIH HHS [CA096694] Funding Source: Medline
- NCRR NIH HHS [P20RR17698] Funding Source: Medline
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Oncogenic Ras mutations are frequently observed in colorectal cancer and participate in neoplastic transformation of intestinal epithelial cells. Accumulating evidence demonstrates the chemopreventive properties of green tea on colon carcinogenesis. Here we investigated the major green tea polyphenol, (-)-epigallocatechin-3-gallate (EGCG), to inhibit proliferation of intestinal epithelial cells (RIE-1) transfected with an inducible Ha-Ras(Val12) cDNA. EGCG inhibited cell proliferation induced by oncogenic Ras and blocked cell cycle transition at G1 phase via inhibition of cyclin D1 expression. The EGCG IC50 was 42 mu M in transformed cells and 81 mu M in non-transformed cells. EGCG also promoted E-cadherin expression, which is downregulated by Ras transformation. This study demonstrates the potential of the natural compound EGCG as an effective adjuvant therapy for colon tumors bearing Ras mutations. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
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