Journal
JOURNAL OF ETHNOPHARMACOLOGY
Volume 106, Issue 3, Pages 364-371Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.jep.2006.01.009
Keywords
Phellinus linteus; anti-inflammatory; heme oxygenase-1; reactive oxygen species; c-jun NH2-terminal kinase; nitric oxide
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This work aimed to elucidate the anti-inflammatory mechanism of the n-BuOH subfraction (PL) prepared from fruiting bodies of Phellinus linteus. PL induced heme oxygenase-1 (HO-1) of the RAW264.7 macrophages in concentration- and time-dependent manner. It suppressed induction of inducible nitric oxide synthase (iNOS) and subsequent production of nitric oxide (NO) through down-regulation of iNOS promoter activity in lipopolysaccharide (LPS)-stimulated macrophages. Zn(II) protoporphyrin IX (ZnPP), a specific inhibitor of HO-1, partly blocked suppression by PL on iNOS promoter activity and NO production, which were elevated in LPS-stimulated macrophages. LPS was able to enhance NO production via reactive oxygen species (ROS) generation, c-Jun NH2-terminal kinase (INK) and c-Jun induction. ZnPP prevented PL from down-regulating ROS generation and JNK activation in LPS-stimulated macrophages. Taken together, PL shows its anti-inflammatory activity via mediation of HO-1 in an in vitro inflammation model. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
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