Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1121, Issue 2, Pages 219-227Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2006.04.073
Keywords
computer optimization/prediction; ternary solvent-strength gradient chromatography; rapid reversed-phase LC analysis; short columns; computer modelling software
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An optimization strategy for ternary solvent-strength gradient elution RP chromatography is described in which a two-dimensional model of gradient time (2 levels) against ternary proportions of organic modifiers (4 levels) was constructed. From the resolution surface the optimum ratio of organic modifiers could be selected. Excellent retention time and acceptable peak width and resolution simulations were obtained. The separation could be further optimized from the same input data by using a standard one-dimensional model in order to optimize for gradient slope, duration and shape. Excellent retention time and acceptable peak width and resolution simulations were obtained (< 1, 2 and 6% error, respectively). (c) 2006 Elsevier B.V. All rights reserved.
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