Journal
SCIENCE
Volume 313, Issue 5785, Pages 337-341Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1126590
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- PHS HHS [206-00139-06] Funding Source: Medline
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Amphiphilic phospholipids were used to direct the formation of biocompatible, uniform silica nanostructures in the presence of Saccharomyces cerevisiae and bacterial cell lines. The cell surfaces organize multilayered phospholipid vesicles that interface coherently with the silica host and help relieve drying stresses that develop with conventional templates. These host structures maintain cell accessibility, addressability, and viability in the absence of buffer or an external fluidic architecture. The cell surfaces are accessible and can be used to localize added proteins, plasmids, and nanocrystals. Prolonged cell viability combined with reporter protein expression enabled stand-alone cell-based sensing.
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