Journal
FEBS LETTERS
Volume 580, Issue 17, Pages 4176-4181Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2006.06.071
Keywords
Chk1; claspin; checkpoint; ATR; proteolysis; DNA damage
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Claspin is involved in ATR-dependent activation of Chk1 during DNA replication and in response to DNA damage. We show that degradation of Claspin by the ubiquitin-proteosome pathway is regulated during the cell cycle. Claspin is stabilized in S-phase but is abruptly degraded in mitosis and is absent from early G, cells in which the phosphorylation of Chk1 by ATR is abrogated. In response to hydroxyurea, UV or aphidicolin, Claspin is phosphorylated in the Chk1-binding domain and its protein levels are increased in an ATR-dependent manner. Thus, the Chk1 pathway is regulated through both phosphorylation of Claspin and its controlled degradation. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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