Journal
FEBS LETTERS
Volume 580, Issue 17, Pages 4105-4113Publisher
WILEY
DOI: 10.1016/j.febslet.2006.06.052
Keywords
visfatin; pre-B-cell colony-enhancing factor; hypoxia; hypoxia-inducible factor-1 alpha; breast cancer cells
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Visfatin has been originally identified as a growth factor for early stage B cells and recently known as an adipokine. Here, we report that hypoxia induces the visfatin mRNA and protein levels in MCF7 breast cancer cells. We also demonstrate that induction of visfatin gene is regulated by hypoxia-inducible factor-1 alpha (HIF-1 alpha). Moreover, 5 '-flanking promoter region of human visfatin gene contains two functional HIF responsive elements (HREs), activating the expression of visfatin. Mutation of these HREs in the visfatin promoter abrogates activation of a luciferase reporter gene driven by visfatin promoter under hypoxia. Taken together, our results demonstrate that visfatin is a new hypoxia-inducible gene of which expression is stimulated through the interaction of HIF-1 with HRE sites in its promoter region. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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