Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 281, Issue 30, Pages 20666-20672Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M512671200
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- NHLBI NIH HHS [HL-071623] Funding Source: Medline
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Thyroid hormones affect cardiac growth and phenotype; however, the mechanisms by which the hormones induce cardiomyocyte hypertrophy remain uncharacterized. Tri-iodo-L-thyronine ( T3) treatment of cultured cardiomyocytes for 24 h resulted in a 41 +/- 5% ( p < 0.001) increase in [ H-3] leucine incorporation into total cellular protein. This response was abrogated by the phosphatidylinositol 3-kinase ( PI3K) inhibitor, wortmannin. Co-immunoprecipitation studies showed a direct interaction of cytosol-localized thyroid hormone receptor TR alpha 1 and the p85 alpha subunit of PI3K. T3 treatment rapidly increased PI3K activity by 52 +/- 3% ( p < 0.005), which resulted in increased phosphorylation of downstream kinases Akt and mammalian target of rapamycin ( mTOR). This effect was abrogated by pretreatment with wortmannin or LY294002. Phosphorylation of p70(S6K), a known target of mTOR, occurred rapidly following T3 treatment and was inhibited by rapamycin and wortmannin. In contrast, phosphorylation of the p85 variant of S6K in response to T3 was not blocked by LY294002, wortmannin, or rapamycin, thus supporting a T3-activated pathway independent of PI3K and mTOR. 40 S ribosomal protein S6, a target of p70(S6K), and 4E-BP1, a target of mTOR, were both phosphorylated within 15 - 25 min of T3 treatment and could be inhibited by wortmannin and rapamycin. Thus, rapid T3-mediated activation of PI3K by cytosolic TR alpha 1 and subsequent activation of the Akt-mTOR-S6K signaling pathway may underlie one of the mechanisms by which thyroid hormone regulates physiological cardiac growth.
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