Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 314, Issue 1-2, Pages 164-169Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2006.05.013
Keywords
beta-glucan; Dectin-1; ELISA; innate immunity; C-type lectin
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Funding
- Wellcome Trust Funding Source: Medline
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beta-Glucans are structural components of fungal cell walls which are involved in the immune recognition of fungal pathogens and possess beneficial immunomodulatory activities in isolated form. Here we have developed a soluble chimeric form of the major mammalian p-glucan receptor, Dectin-1, and demonstrate its application for the detection and characterisation of soluble and insoluble beta-glucans, including fungal particles, using ELISA, flow cytometric and fluorescence-based microscopy assays. (c) 2006 Elsevier B.V. All rights reserved.
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