Journal
PLANT CELL TISSUE AND ORGAN CULTURE
Volume 86, Issue 2, Pages 219-231Publisher
SPRINGER
DOI: 10.1007/s11240-006-9111-5
Keywords
Delta(6)-desaturase; gamma-linolenic acid; C18 fatty acid; genetic manipulation; transgenic Brassica plant
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Genetic manipulation of the oil-yielding crop plants for better oil quality through biotechnological methods is an important aspect of crop improvement. Due to the inherent absence of the triangle(6) stop-desaturase (d6D) function, Brassica juncea, an oil-yielding crop plant, is unable to synthesize gamma-linolenic acid (GLA), a nutritionally important fatty acid although the crop plant synthesizes the precursor fatty acids required for GLA production. Cyanobacterial d6D introduces carbon-carbon double bond onto linoleic acid (C18:2) and alpha-linolenic acid (C18:3) by desaturation processes for production of GLA and octadecatetraenoic acid (OTA) respectively. In the present investigation, d6D coding sequence from Synechocystis sp. PCC6803 was cloned by polymerase chain reaction and introduced into B. juncea through Agrobacterium-mediated transformation technique. Both cytosolic as well as seed-specific expression of d6D were attempted. The transformed plants show production of GLA and OTA in contrast to their absence in the untransformed control plants adducing evidence for introgression and functional expression of the cyanobacterial d6D gene in B. juncea.
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