Journal
MOLECULAR BIOLOGY OF THE CELL
Volume 17, Issue 8, Pages 3446-3455Publisher
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E06-02-0102
Keywords
-
Categories
Funding
- NEI NIH HHS [EY13755, R01 EY013755, R01 EY15227, R01 EY015227] Funding Source: Medline
- NIEHS NIH HHS [P30 ES006096, P30 ES06096] Funding Source: Medline
Ask authors/readers for more resources
The mitogen-activated protein kinase kinase (MEK) kinase 1 (MEKK1) mediates activin B signals required for eyelid epithelium morphogenesis during mouse fetal development. The present study investigates the role of MEKK1 in epithelial wound healing, another activin-regulated biological process. In a skin wound model, injury markedly stimulates MEKK1 expression and activity, which are in turn required for the expression of genes involved in extracellular matrix (ECM) homeostasis. MEKK1 ablation or down-regulation by interfering RNA significantly delays skin wound closure and impairs activation of Jun NH2-terminal kinases, induction of plasminogen activator inhibitor (PAI)-1, and restoration of cell-cell junctions of the wounded epidermis. Conversely, expression of wild-type MEKK1 accelerates reepithelialization of full-thickness skin and corneal debridement wounds by mechanisms involving epithelial cell migration, a cell function that is partially abolished by neutralizing antibodies for PAI-1 and metalloproteinase III. Our data suggest that MEKK1 transmits wound signals, leading to the transcriptional activation of genes involved in ECM homeostasis, epithelial cell migration, and wound reepithelialization.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available