Journal
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
Volume 61, Issue 8, Pages 806-812Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/gerona/61.8.806
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- NIA NIH HHS [R01 AG017768-01A1, AG 17768, K02 AG021626-01, AG 21626, R01 AG017768-03, K02 AG021626-02, R01 AG017768-05, R01 AG017768-02, K02 AG021626-03, K02 AG021626-04, R01 AG017768-04, R01 AG017768, K02 AG021626] Funding Source: Medline
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On the basis of the accelerated age-related effects in type II muscle, we hypothesized that with aging the semimembranosus (type II) muscle would accumulate a greater amount of oxidized proteins compared to proteins in the soleus (type I) muscle. In this study, 3-nitrotyrosine (3-NT) was used as a stable marker of protein oxidative damage. The presence of 3-NT was evaluated in muscles from young adult, old, and very old Fischer 344 rats to provide an indication of the time course of muscle protein oxidative damage. A significant age-associated increase in nitrotyrosine-modified proteins was observed. The modified proteins identified by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry include the sarcoplasmic reticulum Ca+2-ATPase, aconitase, beta-enolase, triosephosphate isomerase, and carbonic anhydrase III. These proteins, involved in metabolism and calcium homeostasis, exhibited an age-dependent increase in 3-NT content in both muscles. However, significant levels of 3-NT modification were present at an earlier age in the semimembranosus muscle.
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