Journal
DEVELOPMENTAL CELL
Volume 11, Issue 2, Pages 159-169Publisher
CELL PRESS
DOI: 10.1016/j.devcel.2006.06.004
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Funding
- NICHD NIH HHS [R01 HD043403, R01 HD43403] Funding Source: Medline
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The regenerative abilities of freshwater planarians are based on neoblasts, stem cells maintained throughout the animal's life. We show that a member of the Brunolike family of RNA binding proteins is critical for regulating neoblasts in the planarian Schmidtea mediterranea. Smed-bruno-like (bruli) mRNA and protein are expressed in neoblasts and the central nervous system. Following bruii RNAi, which eliminates detectable Bruli protein, planarians initiate the proliferative response to amputation and form small blastemas but then undergo tissue regression and lysis. We characterize the neoblast population by using antibodies recognizing SMEDWI-1 and Histone H4 (monomethyl-K20) and cell-cycle markers to label subsets of neoblasts and their progeny. bruli knockdown results in a dramatic reduction/elimination of neoblasts. Our analyses indicate that neoblasts lacking Bruli can respond to wound stimuli and generate progeny that can form blastemas and differentiate; yet, they are unable to-self-renew. These results suggest that Bruli is required for stem cell maintenance.
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