Journal
PLANT AND CELL PHYSIOLOGY
Volume 47, Issue 8, Pages 1175-1181Publisher
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcj086
Keywords
actin filament; dynamin; microtubule; microtubule-associated protein (MAP); NtDRP3; tobacco BY-2 cell
Categories
Ask authors/readers for more resources
We purified an 84 kDa polypeptide from the MAP (microtubule-associated protein) fraction of tobacco BY-2 cultured cells. LC/MS/MS (liquid chromatography-tandem mass spectrometry) analysis revealed that this polypeptide is a tobacco homolog of AtDRP3 (Arabidopsis thaliana dynamin-related protein 3). Electron microscopy revealed that NtDRP3 (Nicotiana tabacum dynamin-related protein 3) assembles to form a filamentous structure. When GDP was added to the NtDRP3 fraction, the filaments disappeared and many particles appeared. Biochemical analysis revealed that NtDRP3 could bind to and bundle both microtubules and actin filaments in vitro.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
