Selection of the cutaneous intraepithelial γδ+ T cell repertoire by a thymic stromal determinant

Intraepithelial lymphocytes constitute a group of T cells that express mainly monospecific or oligoclonal T cell receptors (TCRs). Like adaptive TCR alpha beta(+) T cells, intraepithelial lymphocytes, a subset enriched in TCR gamma delta(+) T cells, are proposed to be positively selected by thymically expressed self agonists, yet no direct evidence for this exists at present. Mouse dendritic epidermal T cells are prototypic intraepithelial lymphocytes, displaying an almost monoclonal TCR gamma delta(+) repertoire. Here we describe an FVB substrain of mice in which this repertoire was uniquely depleted, resulting in cutaneous pathology. This phenotype was due to failure of dendritic epidermal T cell progenitors to mature because of a heritable defect in a dominant gene used by the thymic stroma to 'educate' the natural, skin- associated intraepithelial lymphocyte repertoire to be of physiological use.