Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 51, Issue 1, Pages 25-29Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2006.05.013
Keywords
perfluorinated surfactants; DPPC; DSC; liposome; membrane fluidity
Funding
- NIEHS NIH HHS [ES 012475, K25 ES012475-01A1, K25 ES012475, K25 ES012475-02] Funding Source: Medline
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Perfluorooctane-I-sulfonic acid (PFOS) is emerging as an important persistent environmental pollutant. To gain insight into the interaction of PFOS with biological systems, the mixing behavior of dipalmitoylphosphatidylcholine (DPPC) with PFOS was studied using differential scanning calorimetry (DSC) and fluorescence anisotropy measurements. In the DSC experiments the onset temperature of the DPPC pretransition (T-p) decreased with increasing PFOS concentration, disappearing at X-DPPC <= 0.97. The main DPPC phase transition temperature showed a depression and peak broadening with increasing mole fraction of PFOS in both the DSC and the fluorescence anisotropy studies. From the melting point depression in the fluorescence anisotropy studies, which was observed at a concentration as low as 10 mg/L, an apparent partition coefficient of K = 5.7 x 10(4) (mole fraction basis) was calculated. These results suggest that PFOS has a high tendency to partition into lipid bilayers. These direct PFOS-DPPC interactions are one possible mechanism by which PFOS may contribute to adverse effects, for example neonatal mortality, in laboratory studies and possibly in humans. (c) 2006 Elsevier B.V. All rights reserved.
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