Journal
MOLECULAR THERAPY
Volume 14, Issue 2, Pages 245-254Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymthe.2006.04.005
Keywords
self-inactivating gammaretroviral vector; embryonic stem cells; hematopoietic stem cells; differentiation; stable transgene expression
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Funding
- NCRR NIH HHS [R24 RR016209, R24RR16209] Funding Source: Medline
- NHLBI NIH HHS [R01 HL065519-05, R01 HL066305-04, R01 HL065519, R01 HL066305, R01HL65519, R01 HL066305-05, R01 HL065519-06, R01HL66305] Funding Source: Medline
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Unlike conventional gammaretroviral vectors, the murine stem cell virus (MSCV) can efficiently express transgenes in undifferentiated embryonic stem cells (ESCs). However, a dramatic extinction of expression is observed when ESCs are subjected to in vitro hematopoietic differentiation. Here we report the construction of a self-inactivating vector from MSCV, MSinSB, which transmits an intron embedded within the internal transgene cassette to transduced cells. The internal transgene transcriptional unit in MSinSB comprises the composite cytomegalovirus immediate early enhancer-chicken beta-actin promoter and associated 51 splice site positioned upstream of the natural 31 splice site of the gammaretroviral envelope gene, and is configured such that the transgene translational initiation sequence is coincident with the envelope ATG. MSinSB could be produced at titers approaching 10(6) transducing units/ml and directed higher levels of transgene expression in ESCs than a splicing-optimized MSCV-derived vector, MSGV1. Moreover, when transduced ESCs were differentiated into hematopoietic cells in vitro, MSinSB remained transcriptionally active in greater than 90% of the cells, whereas MSGV1 expression was almost completely shut off. Persistent high-level expression of the MSinSB gammaretroviral vector was also demonstrated in murine bone marrow transplant recipients and following in vitro myelomonocytic differentiation of human CD34(+) cord blood stem/progenitor cells.
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