Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 72, Issue 8, Pages 5637-5642Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.00739-06
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The insertion element InsTip alpha was constructed to generate protein expression data. It randomly fuses the TetR-inducing peptide Tip to the affected reading frame. Fusion protein expression is quantified by Tet-regulated reporter gene expression. The expression patterns of tagged Escherichia coli genes fully agree with published data from transcriptional fusions or microarrays, validating the Tip tag approach.
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