Journal
DEVELOPMENT
Volume 133, Issue 15, Pages 2925-2935Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.02450
Keywords
zebrafish; telencephalon; eye field; forebrain; Rx3
Categories
Funding
- Biotechnology and Biological Sciences Research Council [BB/E005403/1] Funding Source: researchfish
- Medical Research Council [G9900989B] Funding Source: researchfish
- Biotechnology and Biological Sciences Research Council [BB/E005403/1] Funding Source: Medline
- BBSRC [BB/E005403/1] Funding Source: UKRI
Ask authors/readers for more resources
Anteroposterior patterning of the vertebrate forebrain during gastrulation involves graded Wnt signaling, which segregates anterior fields ( telencephalon and eye) from the diencephalon. How the telencephalic and retinal primordia are subsequently subdivided remains largely unknown. We demonstrate that at late gastrulation the Paired-like homeodomain transcription factor Rx3 biases cell specification choices towards the retinal fate within a population of bipotential precursors of the anterior forebrain: direct cell tracing demonstrates that retinal precursors acquire a telencephalic fate in embryos homozygous for the rx3-null allele ckh(ne2611), characterized by an enlarged telencephalon and a lack of eyes. Chimera analyses further indicate that this function of Rx3 is cell autonomous. Transfating of the eye field in the absence of Rx3 function correlates with a substantial posterior expansion of expression of the Wnt antagonist Tlc and the winged-helix transcription factor Foxg1. These results suggest that the process segregating the telencephalic and eye fields is isolated from diencephalic patterning, and is mediated by Rx3.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available