Journal
APPLIED RADIATION AND ISOTOPES
Volume 64, Issue 8, Pages 901-905Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.apradiso.2006.03.002
Keywords
cellular uptake; cellular retention; protein interactions; kinetics
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This report describes an automated method for the measurements of cellular retention and uptake of radiolabeled proteins interacting with cell-surface receptors on intact cancer cells. A complete uptake and retention measurement was performed in one cell dish using a rotating radioimmunoassay (RIA) principle. Compared to common manual measurements, rotating RIA saved both labor time and reagents and provided real-time binding traces with superior time-resolution. The rotating RIA retention profiles for different interactions agreed with retention times reported in the literature. (c) 2006 Elsevier Ltd. All rights reserved.
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