Journal
JOURNAL OF IMMUNOLOGY
Volume 177, Issue 3, Pages 1708-1720Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.177.3.1708
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Funding
- NCI NIH HHS [R01 CA090436-01A2, CA90436, R01 CA090436] Funding Source: Medline
- NIGMS NIH HHS [R01 GM056322, GM56322] Funding Source: Medline
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T cell polarization toward and within the cellular interface with an APC is critical for effective T cell activation. The Rho family GTPase Cdc42 is a central regulator of cellular polarization. Using live-cell imaging, we characterized the spatiotemporal patterns of Cdc42 activity and their physiological regulation. Using three independent means of experimental manipulation of Cdc42 activity, we established that Cdc42 is a critical regulator of T cell actin dynamics, TCR clustering, and cell cycle entry. Using quantification of three-dimensional data, we could relate distinct spatiotemporal patterns of Cdc42 activity to specific elements of T cell activation. This result suggests that Cdc42 activity in specific locations at specific times is most critical for its function in T cell activation.
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