Journal
TRENDS IN BIOTECHNOLOGY
Volume 24, Issue 8, Pages 364-371Publisher
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tibtech.2006.06.008
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Functional and structural studies of membrane proteins usually require overexpression of the proteins in question. Often, however, the 'trial and error' approaches that are mainly used to produce membrane proteins are not successful. Our rapidly increasing understanding of membrane protein insertion, folding and degradation means that membrane protein overexpression can be more rationalized, both at the level of the overexpression host and the overexpressed membrane protein. This change of mindset is likely to have a significant impact on membrane protein research.
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