Journal
JOURNAL OF SEPARATION SCIENCE
Volume 29, Issue 12, Pages 1720-1732Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200600151
Keywords
nanomaterials; polymers; proteins; protein aggregates; supramolecular assemblies
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This review summarizes developments and applications of flow and thermal field-flow fractionation (FFF) in the areas of macromolecules and supramolecular assemblies. In the past 10 years, the use of these FFF techniques has extended beyond determining diffusion coefficients, hydrodynamic diameters, and molecular weights of standards. Complex samples as diverse as polysaccharides, prion particles, and block copolymers have been characterized and processes such as aggregation, stability, and infectivity have been monitored. The open channel design used in FFF makes it a gentle separation technique for high- and ultrahigh-molecular weight macromolecules, aggregates, and self-assembled complexes. Coupling FFF with other techniques such as multiangle light scattering and MS provides additional invaluable information about conformation, branching, and identity.
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