Journal
BIOTECHNOLOGY LETTERS
Volume 28, Issue 15, Pages 1199-1207Publisher
SPRINGER
DOI: 10.1007/s10529-006-9078-7
Keywords
5-enolpyruvyl-3-phosphoshikimate synthase (EPSPS); cotton; glyphosate; selectable marker; transformation
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The most economically significant Chinese cotton cultivar (Gossypium hirsutum L. cv. Zhongmian 35) was transformed via Agrobacterium tumefaciens-mediated DNA transfer. The aroA-M1 gene that confers resistance to the glyphosate was fused with a chloroplast-transit peptide of Arabidopsis thaliana 5-enolpyruvyl-3-phosphoshikimate synthase (ASP) and expressed in cotton plants under the control of a CaMV35S promoter. Transgenic plants were directly selected on medium containing glyphosate. Thirty-four independent transgenic lines were obtained after selection, giving a maximal 1.9% transformation frequency. The integration and expression of the aroA-M1 gene in T-0 plants and T-1 progeny were confirmed using DNA hybridization, Western blot and PCR techniques. An increased resistance of T-0 and T-1 transgenic plants towards glyphosate was also observed.
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